Human Gene WIPF2 (uc002hug.1)
Description: hom*o sapiens WAS/WASL interacting protein family, member 2 (WIPF2), mRNA. RefSeq Summary (NM_133264): This gene encodes a WASP interacting protein (WIP)-related protein. It has been shown that this protein has a role in the WASP-mediated organization of the actin cytoskeleton and that this protein is a potential link between the activated platelet-derived growth factor receptor and the actin polymerization machinery. [provided by RefSeq, Jul 2008]. Transcript (Including UTRs) Position:hg19 chr17:38,375,574-38,438,439Size:62,866Total Exon Count:8Strand:+ Coding Region Position:hg19 chr17:38,412,712-38,434,477Size:21,766Coding Exon Count:7
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Sequence and Links to Tools and Databases
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Comments and Description Text from UniProtKB
ID: WIPF2_HUMAN DESCRIPTION: RecName: Full=WAS/WASL-interacting protein family member 2; AltName: Full=WASP-interacting protein-related protein; AltName: Full=WIP- and CR16-hom*ologous protein; AltName: Full=WIP-related protein; FUNCTION: Plays an active role in the formation of cell surface protrusions downstream of activated PDGFB receptors. Plays an important role in actin-microspike formation through cooperation with WASL. May cooperate with WASP and WASL to induce mobilization and reorganization of the actin filament system. SUBUNIT: Interacts with WASL and WASP, and this interaction results in cytoplasmic relocation of these two proteins along actin filaments. Interacts with NCK2 resulting in the localization to sites of focal adhesions. No interaction was seen with WASF2 and WASF3. INTERACTION: P62993:GRB2; NbExp=3; IntAct=EBI-2850112, EBI-401755; SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Note=Localized to stress fibers and bundles of actin filaments. TISSUE SPECIFICITY: Expressed mainly in brain, colon, lung and stomach (at protein level). Ubiquitously expressed, with high expression in brain, kidney, lung, and placenta. MISCELLANEOUS: Access to the profilin-binding site is masked in the full-length protein. SIMILARITY: Belongs to the verprolin family. SIMILARITY: Contains 1 WH2 domain. SEQUENCE CAUTION: Sequence=AAQ15232.1; Type=Frameshift; Positions=373; |
Primer design for this transcript
Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA. Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence. To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3 |
RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
Highest median expression: 13.25 RPKM in Brain - Frontal Cortex (BA9) Total median expression: 368.34 RPKM View in GTEx track of Genome BrowserView at GTEx portalView GTEx Body Map |
mRNA Secondary Structure of 3' and 5' UTRs
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have. |
Protein Domain and Structure Information
InterPro Domains: Graphical view of domain structure IPR003124 - WH2_dom Pfam Domains: ModBase Predicted Comparative 3D Structure on Q8TF74
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
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Gene Ontology (GO) Annotations with Structured Vocabulary
Molecular Function: GO:0003779 actin binding GO:0005515 protein binding Biological Process: Cellular Component: |
Descriptions from all associated GenBank mRNAs
AK289779 - hom*o sapiens cDNA FLJ78696 complete cds, highly similar to hom*o sapiens WIRE protein (WIRE), mRNA. AK311544 - hom*o sapiens cDNA, FLJ18586. AL834239 - hom*o sapiens mRNA; cDNA DKFZp547N082 (from clone DKFZp547N082). BC025965 - hom*o sapiens cDNA clone IMAGE:5458710. AK315836 - hom*o sapiens cDNA, FLJ79485 complete cds, highly similar to WIP-related protein. AK290301 - hom*o sapiens cDNA FLJ77749 complete cds, highly similar to hom*o sapiens WIRE protein (WIRE), mRNA. AK055057 - hom*o sapiens cDNA FLJ30495 fis, clone BRAWH2000323, moderately similar to WIP-related protein. BC065551 - hom*o sapiens WAS/WASL interacting protein family, member 2, mRNA (cDNA clone MGC:70467 IMAGE:6044222), complete cds. AF370396 - hom*o sapiens PP10631 mRNA, complete cds. AK289578 - hom*o sapiens cDNA FLJ75102 complete cds, highly similar to hom*o sapiens WIRE protein (WIRE), mRNA. LF326319 - JP 2014500723-A/133822: Polycomb-Associated Non-Coding RNAs. JC506674 - Sequence 42 from Patent EP2733220. JC737786 - Sequence 42 from Patent WO2014075939. JC506661 - Sequence 29 from Patent EP2733220. JC737773 - Sequence 29 from Patent WO2014075939. JC506688 - Sequence 56 from Patent EP2733220. JC737800 - Sequence 56 from Patent WO2014075939. JC506666 - Sequence 34 from Patent EP2733220. JC737778 - Sequence 34 from Patent WO2014075939. MA561896 - JP 2018138019-A/133822: Polycomb-Associated Non-Coding RNAs. AB043786 - hom*o sapiens mRNA for WICH, complete cds. AJ431177 - hom*o sapiens mRNA for WIRE protein. AK026913 - hom*o sapiens cDNA: FLJ23260 fis, clone COL05804, highly similar to HSU90911 Human clone 23652 mRNA sequence. BC043408 - hom*o sapiens WIRE protein, mRNA (cDNA clone IMAGE:6064801), partial cds. U90911 - Human clone 23652 mRNA sequence. JD164812 - Sequence 145836 from Patent EP1572962. JD450065 - Sequence 431089 from Patent EP1572962. JD040589 - Sequence 21613 from Patent EP1572962. JD091033 - Sequence 72057 from Patent EP1572962. JD126466 - Sequence 107490 from Patent EP1572962. JD352339 - Sequence 333363 from Patent EP1572962. JD036939 - Sequence 17963 from Patent EP1572962. JD538693 - Sequence 519717 from Patent EP1572962. JD039427 - Sequence 20451 from Patent EP1572962. JD133503 - Sequence 114527 from Patent EP1572962. JD204224 - Sequence 185248 from Patent EP1572962. JD306507 - Sequence 287531 from Patent EP1572962. JD390372 - Sequence 371396 from Patent EP1572962. JD207510 - Sequence 188534 from Patent EP1572962. JD036865 - Sequence 17889 from Patent EP1572962. JD412562 - Sequence 393586 from Patent EP1572962. JD422579 - Sequence 403603 from Patent EP1572962. JD263105 - Sequence 244129 from Patent EP1572962. JD300322 - Sequence 281346 from Patent EP1572962. JD530678 - Sequence 511702 from Patent EP1572962. JD547513 - Sequence 528537 from Patent EP1572962. DQ586635 - hom*o sapiens piRNA piR-53747, complete sequence. JD071848 - Sequence 52872 from Patent EP1572962. JD137061 - Sequence 118085 from Patent EP1572962. JD372813 - Sequence 353837 from Patent EP1572962. JD186473 - Sequence 167497 from Patent EP1572962. JD019112 - Sequence 136 from Patent EP1572962. JD034563 - Sequence 15587 from Patent EP1572962. BC019706 - hom*o sapiens cDNA clone IMAGE:4513687, partial cds. AK055025 - hom*o sapiens cDNA FLJ30463 fis, clone BRACE2009517. BC047435 - hom*o sapiens cDNA clone IMAGE:5313516. BC064504 - hom*o sapiens cDNA clone IMAGE:6537819, partial cds. JD148351 - Sequence 129375 from Patent EP1572962. JD249926 - Sequence 230950 from Patent EP1572962. JD511594 - Sequence 492618 from Patent EP1572962. JD319667 - Sequence 300691 from Patent EP1572962. JD447282 - Sequence 428306 from Patent EP1572962. JD149213 - Sequence 130237 from Patent EP1572962. JD149215 - Sequence 130239 from Patent EP1572962. JD054482 - Sequence 35506 from Patent EP1572962. JD106509 - Sequence 87533 from Patent EP1572962. JD044561 - Sequence 25585 from Patent EP1572962. DQ598333 - hom*o sapiens piRNA piR-36399, complete sequence. |
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO) Protein Q8TF74 (Reactome details) participates in the following event(s): R-HSA-2197691 Activation of WASP/N-WASP by WIP family and SH3 domain proteins |
Alternate Gene Symbols: A8K0L3, NM_133264, NP_573571, PP10631, Q658J8, Q71RE1, Q8TE44, Q8TF74, WICH, WIPF2_HUMAN, WIRE UCSC ID: uc002hug.1 RefSeq Accession: NM_133264 Protein: Q8TF74 (aka WIPF2_HUMAN) CCDS: CCDS11364.1 |
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